Description
Principle of the assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with ORM2. Standards or samples are added to the appropriate microtiter plate wells with Horseradish Peroxidase (HRP) conjugated antibody preparation specific for ORM2. The competitive inhibition reaction is launched between with pre-coated ORM2 and ORM2 in samples. A substrate solution is added to the wells and the color develops in opposite to the amount of ORM2 in the samples. The color development is stopped and the intensity of the color is measured